The HI84533-61 is a 230 mL bottle of formol base reagent that is used for the determination of formol number in wines and fruit juices.The HI84533-61 formol base reagent is required for analysis because it reacts with the amino acids and nitrogenous compounds in the sample. This reaction frees hydrogen ions in an amount proportional to the available nitrogen. The titration neutralizes the free hydrogen ions and provides an accurate determination of total assimilable nitrogen.The content of amino acids and other nitrogen compounds in wine or fruit juice is determined by an acid base titration to a fixed pH endpoint and expressed as total assimilable nitrogen. The determination of formol number is possible only after neutralization of the titratable acids present by a pH adjustment with HI84533-62 reagent, followed by the blockage of the amino groups by reaction with the HI84533-61 formol base reagent. The titration is then performed with the HI84533-50 titrant solution.Replacement reagents required to operate the HI84533 include:
| Low Range – 30.0 to 400.0 mg/L as N | High Range – 300 to 1000 mg/L as N | |
| Pump calibration standard | HI84533-55 | HI84533-55 |
| Titrant solution | HI84533-50 | HI84533-50 |
| pH adjustment reagent | HI84533-62 | HI84533-62 |
| Formol base reagent | HI84533-61 | HI84533-61 |
| Additional reagent | HI84533-60 | HI84533-60 |
